Potential recombinant plasmids were used to transform ultracompetent cells of Escherichia coli DH5[alpha] strain by the heat shock method.

The capsid fragment was cloned into a pPCR-Script Amp SK(+) vector and transformed into XL10-Gold Kan ultracompetent cells, using the Stratagene PCR-Script Amp Cloning Kit (Stratagene) according to the manufacturer's instructions.

MVP of both cabal and tuner is ultracompetent Violet Newstead, Lily Tomlin's signature role to which Allison Janney brings her "West Wing"-honed professionalism along with unexpected showbiz pizzazz.

I knew at the time that I never felt safe in my house, but didn't connect that feeling with my passion for stories about ultracompetent observers, experts at concealment and control of their emotions, men who survived by stealth and vigilance and grim moxie.

Each was cloned into pPCR-Script by blunt-end ligation and transformed into XL-10 Gold Ultracompetent cells.

Each ligation product (2 [micro]L) was transformed into XL-II Ultracompetent Sure Cells (Stratagene).

Double stranded DNA was cloned into pSTBlue-1 by blunt end ligation and transformed into Nova Blue ultracompetent cells.

The crew consists of Bowman (Carrie-Anne Moss), the ultracompetent mission commander, Gallagher (Val Kilmer), flight engineer and Bowman's soul mate; Burchenal (Tom Sizemore), lead scientist; Santen (Benjamin Bratt), copilot and resident tough guy; Pettengil (Simon Baker), the very young agricultural specialist; and Chantilas (Terence Stamp), an aging space cadet who's still looking for signs of God up in the heavens after many journeys into the beyond.