starting buffer


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Noun1.starting buffer - buffer solution at the start of a reaction
buffer solution - a solution containing a buffer
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Unadsorbed proteins (fraction D1) were eluted with the starting buffer. Adsorbed proteins eluted with the buffer 2 (the starting buffer with 0.2 M NaCl) were collected as fraction D2, and adsorbed proteins eluted with the buffer 3 (the starting buffer with 1 M NaCl) were collected as fraction D3.
Before this, the troponin-free serum was mixed 1:1 with starting buffer containing 50 mmol/L Tris, 500 mmol/L NaCl, pH 7.0, and is subsequently referred to as "normal serum".
After removal of the unadsorbed fraction (Dl), adsorbed fractions (D2 and D3) were eluted with starting buffer containing 0.2 and 1 M NaCl, respectively.