Biotechnology Principles and Processes

• This paper contains 60 multiple choice questions. Each question has four choices (a), (b), (c) and
(d), out of which ONLY ONE is correct. (Mark only one choice).
Marks: 60 × 4 = 240 Negative Marking: (–1)
(1)The enzymes that cuts specifically recognition (c) Ligase chain reaction (d) polymerase chain
sites in the DNA is known as reaction
(a) DNA ligase (b) DNA Polymerase (10) The enzyme that cleaves DNA at specific
(c) Reverse transcriptase (d) Restriction sites, producing sticky ends is called
endonuclease (a) Restriction endonuclease (b) Cleaving enzyme
(2) DNA can be introduced into any cell by (c) Lysing enzyme (d) Exonuclease
(a) Injection (b) being complexed with Ca salts (11) Which of the Following is a genetic vector?
(c) gel electrophoresis (d) being placed along with (a) Plasmid (b) Phage (c) Cosmid (d) All of these
(3) Ability of a plant or animal cell to repeatedly (12) Restriction endonucleases are used in genetic
divide & differentiate into a complete organism is:- engineering because -
(a) cloning (b) DNA finger printing (a) They can degrade harmful proteins
(c) cellular totipotency (d) mitosis (b) They can join DNA fragments
(4) Restriction endonuclease is also known as - (c) They can cut DNA at specific base sequences
(a) molecular glue (b) DNA ligase (d) They can cut DNA at variable sites
(c) DNA Polymerase (d) molecular scissors (13) Ideal host for the amplification of DNA
(5) Extra chromosomal small cirular double molecules is
stranded DNA molecule in a bacterial cell is (a) Viruses (b) Plants (c) Bacteria (d) Animals
stranded DNA molecule in bacterial cell is (14) Ti Plasmid naturally occurs in
(a) Plastid (b) Plasmid (a) Agro bacterium (b) Corynebacterium
(c) Mitochondrion (d) Chloroplast (c) Staphylococcus (d) Vibrio
(6) Introduction of foreign genes into plant or (15) The sticky ends of Fragmented DNA
animal cells using micropipettes is molecules are made up of
(a) Electroporation (a) calcium salts (b) endonuclease
(b) Chemical - mediated gene transfer (c) unpaired bases (d) methyl groups
(c) microinjection (d) Particle gun (16) Which of the following are the essential
(7) Which one of the following is related with requirements for recombination?
genetic engineering? (a) Single stranded DNA (b) DNA ligase
(a) Mulations (b) Ribosomes (c) DNA Polymerase I (d) All of the above
(c) Mitochondria (d) Plasmids (17) The Plasmid derived from E.Coli is
(8) In bacteria, genes for antibiotic resistance are (a) PBR327 (b)PBR322 (c) both a above (d) None
usually located in (18) Ti Plasmid is useful in
(a) Plasmids (b) Cytoplasm (a) bringing new genes into animal cells
(c) Mitochondria (d) Nucleus (b) bringing new genes into plant cells
(9) A technique used to make numerous copies of a (c) to nearly any sites on a chromosome
specific segment of DNA quickly and accurately (d) bringing tumour cells into plant cells
(a) Translation (b) transcription (19) Many copies of a DNA molecule in a test tube
are procurred by
 Biotechnology Principles and Processes
• This paper contains 60 multiple choice questions. Each question has four choices (a), (b), (c) and
(d), out of which ONLY ONE is correct. (Mark only one choice).
Marks: 60 × 4 = 240 Negative Marking: (–1)
(a) Polymerase chain reaction (PCR) (27) Which one of the following pairs is correctly
(b) Molecular chain reaction (MCR) matched?
(c) Ephemeral chain reaction (ECR) (a) RNA polymerase - RNA primer
(d) All of these (b) Restrication enzymes - Genetic engineering
(20) BamHI, ECoRI, SalI are the types of (c) Centeral dogma - codon
(a) restriction endonucleasses (d) okazaki fragments - splicing
(b) restraction endoxidases (28) Plasmids are autonomously replicating mini
(c) restriction exonucleases chromosomes found in......
(d) restriction polymerases (a) Bachterio phage lambda
(21) Retro viruses have genetic material which is (b) Leishmania donovani
(a) DNA (b) RNA (c) both DAN & RNA (d) proteins (c) Escherichia coli
(22) Genetic engineering is possible because (d) Paramoecium caudatum
(a) the phenomenon of transduction in bacteria is (29) Improvement of genotype of an organism by
well understood addition of some foreigm gene is......
(b) we can see DNA by electron microscope (a) genetic diversity (b) gene handing
(c) we can cut DNA at specific sites by endonucleases (c) tissue culture (d) genetic engineering
like DNAase I (30) Two bacteria found to be very useful in
(d) restriction endonuclease purified from bacteria genetic engineering experiments are......
can be used in vitro (a) Nitrosomonas and Klebsiella
(23) Plasmids are the suitable vectors for genetic (b) Escherichia and Agrobacterium
cloning as..... (c) Nitrobacter and Azotobacter
(a) they are indispensable (d) Rhizobium and Diplococcus
(b) they are self-replicating units (31) Restriction enzymes are isolated chiefly
(c) they are essential for bacterial reproduction from.......
(d) None of the above (a) Algae (b) Fungi (c) Protozoans (d) Prokaryotes
(24) Which of the following is used in genetic (32) There are special proteins that help to open up
engineering ? DNA double helix in front of the
(a) Restrication endonuclease (b) Mycobacterium replication work, these proteins are........
(c) Entamoeba (d) Pepsin (a) DNA gyrase (b) DNA polymerase I
(25) The first hormone artificially produced by (b) DNA ligase (d) DNA topoisomerase
culturing bacteria is______ (33) Technology which uses living components for
(a) Insulin (b) thyroxin the welfare of human being is.....
(c) Testosterone (d) Adrenaline (a) Biology (b) Botany
(26) When the number of genes increases in (C) Bioinformatics (D) Biotechnology
response to some signal the effect is called..... (34) Which prosess is involved in making bread
(a) gene dosage (b)Gene pool cheese, beer and wine?
(c) gene amplification (d) gene freaquency (a) Respiration / hydrolysis (B) Degradation
 Biotechnology Principles and Processes
• This paper contains 60 multiple choice questions. Each question has four choices (a), (b), (c) and
(d), out of which ONLY ONE is correct. (Mark only one choice).
Marks: 60 × 4 = 240 Negative Marking: (–1)
(C) Fermentation (D) Decomposition (a) origin of replication (b) cloning site
(35) EFB stands for ........ (c) origin of restriction (d) selectable marker
(a) European Foundation of Biotechnology (43) Ti Plasmid is a cloning vector which works
(B) European Foundation of Biology with
(c) European Foundation of Biotechnology (a) All the plants (b) Dicots only
(d) European Foundation of Biology (c) Monocots only (d) Thallophytes only
(36) The organism who’s gene have been (44) During which of the following techniques host
artificially altered for desired effect is called as....... cells are exposed to pulse of high voltage current?
(a) genetically mutant organism (b) gene transfer (a) Electroporation (b) Particle Bombardments
(c) genetically modified organism (d) Genetically (c) Micro injection (d) lipofection
transferred organism (45) Particle bombardment technique is also known
(37) The sequence of DNA that reads the same as .....
backward and forward across the double strand (a) Lipofection (b) Electroporation
is........ (c) Biolistic (d) Micro injection
(a) Recipient sequence (B) palindromic sequence (46) Which enzyme is used to break the membrane
(c) Replicate sequence (d) origin sequence to relase plant DNA?
(38) How many restriction enzymes are known to (a) Lysozyme (b) Chitinase
be isolated? (c) Cellulose (d) All the above
(a) more than 800 (b) more than 700 (47) Which enzyme is used to break the membrane
(c) more than 600 (d) more than 900 to release animal DNA?
(39) Which of the following step is necessary part (a) Lysozyme (b) chitinase
of DNA recombination technology? (c) Celluose (d) All the above
(a) Insertion of DNA fragment into vector (48) Which is the first step in the process
(b) Insertion of vector into Bacteria recombinant DNA technology?
(c) multiplication of the clones containing the (a) denaturing of DNA (b) Annealing of DNA
recombination molecule (c) Isolation of Donor DNA (d) Down streaming
(d) All the above (49) Which primers are used in annealing during
(40) Restriction enzymes belong to which class of amplification of gene?
enzymes? (a) Reverse primers (b) Forward primers
(a) Nucleolase (b) Exonucleases (c) Oligonucleotide primer (d) Internal primers
(c) Nucleases (d) Endonucleases (50) What is temperature required for annealing of
(41) A sequence of in a genome at which DNA molecule?
replication is intiated in ......... (a) 50-650 C (b) 30-350 C
(a) origin of replication (b) selectable marker (c) 40-450 C (d) 20-250 C
(c) cloning site (d) origin of restriction (51) Which of the following is related with genetic
(42) Genes which helps in the growth of engineering?
transformants are ..... (a) Breeding (b) somatic hybridization
 Biotechnology Principles and Processes
• This paper contains 60 multiple choice questions. Each question has four choices (a), (b), (c) and
(d), out of which ONLY ONE is correct. (Mark only one choice).
Marks: 60 × 4 = 240 Negative Marking: (–1)
(c) mutation (d) Transgenic (c) animals like bulls for farm work as they have
(52) What is C - DNA? super power.
(a) circular DNA (d) transgenic mice for testing safety of polio
(b) Cloned DNA Vaccine before use in humans.
(c) DNA produced from reverse transcription of (57) Match the following and choose the correct
RNA combination from the option given...
(d) Cytoplasmic DNA Column I Column II
(53) Which of the bollowing statement is incorrect? (a) Escherichia coli 1 - nif gene
(a) cosmid contains gene coding for viral protein (b) Rhizobium meliloti 2 - digestion of
(b) cosmid relpicates like plasmids hydrocarbons of crude oil
(c) cosmid has antibioticresistant marker gene (c) Bacilius thuringiensis 3 - human insulin
(d) cos sit has 12 bases helping to join complete production
genome to make it circular. (d) Pseudomonas putida 4 - Biocontrol of
(54) Gel electrophoresis is used for— fungal disease
(a) Isolation of DNA molecule 5 - biodegradable insecticide
(b) Cutting of DNA in to fragments (a) A = 3, B = 1, C = 5, D = 4
(c) Separation of DNA fragments according to (b) A = 1, B = 2, C = 3, D = 4,
their size (c) A = 2, B = 1, C = 3, D = 4
(d) Construction of recombinant DNA by joining (d) A = 4, B = 3, C = 1, D = 2
with cloning Vector (e) A = 3, B = 1, C = 5, D = 2
(55) Which one of the following Palindromic base (58) Find the incorrect statement
sequence in DNA can be easily cut at about the (a) Gene therapy is a genetic engineering technique
middle by some Particular restriction enzyme? used to treat disease at molecular level by replacing
(a) 5’ ________ GATATG ________ 3’ defective genes with normal genes.
3’ ________ CTACTA ________ 5’ (b) Calcitonin is a medically useful recombinant
(b) 5’ ________ GAATTC ________ 3’ product in the treatment of infertility
3’ ________ CTTAAG ________5’ (c) Bt toxin is a Biodegradable insecticide obtained
(c) 5’ ________ CACGTA _______ 3’ from Bacillis thuringiensis
3’ ________ CTCAGA _______ 5’ (d) Trichoderma sp. is a biocontrol agent for fungal
(d) 5’________ CGTTCG _______ 3’ diseases of plants
3’ ________ ATGGTA ________ 5’ (e) Totipotency is the potential ability of a cell to
(56) Genetic engineering has been successfully develop into a complete plant
used for producing ___ (59) Production of a human protein in bacteria
(a) transgenic models for studying new treatments genetic engineering is possible because
for Certain cardiac diseases. (a) bacterial cell can carry out the RNA splicing
(b) transgenic Cow - Rosie which produces high fat reactions
milk for making ghee.
 Biotechnology Principles and Processes
• This paper contains 60 multiple choice questions. Each question has four choices (a), (b), (c) and
(d), out of which ONLY ONE is correct. (Mark only one choice).
Marks: 60 × 4 = 240 Negative Marking: (–1)
(b) the human chromosome can replicate in
bacterial cell
(c) the mechanism of gene regulation is identical in
human and bacteria
(d) The genetic code is universal
(60) The basis of DNA finger printing is
(a) The double helix
(b) Errors in base sequence
(c) Polymorphism in sequence
(d) DNA replication
(e) DNA Coiling