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Radiochemical Assay of Diamine Oxidase

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Polyamine Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 79))

Abstract

Diamine oxidase activity (E.C. 1.4.3.6) can be measured by a modification of the method first reported by Okuyama and Kobayashi (1), as developed by Trydingetal. (2–4). Oxidation of [1,4-14C]putrescine by diamine oxidase leads to the formation of γ-[14C]-aminobutylaldehyde, which rapidly and spontaneously undergoes an internal cyclization to form the internal aldimine ring compound, ΔA1-[14C]pyrroline (Fig. 1). The pyrroline may undergo further spontaneous polymerization (5). Unlike putrescine, which is very soluble in water, Δ1-pyrroline and its polymers are soluble in toluene at alkaline pH and may be easily separated from the substrate by solvent extraction. This assay system has been extensively reviewed (6–10).

Fates of putrescine in biological mixtures.

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Author information

Authors and Affiliations

  1. Headache Group, Institute of Neurology, London, UK

    R. James Storer

  2. Department of Immunology, Women’s and Children’s Hospital, North Adelaide, Australia

    Antonio Ferrante

Authors
  1. R. James Storer
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  2. Antonio Ferrante
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Editor information

Editors and Affiliations

  1. King’s College, London, UK

    David M. L. Morgan

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© 1998 Humana Press Inc.

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Storer, R.J., Ferrante, A. (1998). Radiochemical Assay of Diamine Oxidase. In: Morgan, D.M.L. (eds) Polyamine Protocols. Methods in Molecular Biology™, vol 79. Humana Press. https://doi.org/10.1385/0-89603-448-8:91

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  • DOI: https://doi.org/10.1385/0-89603-448-8:91

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-448-8

  • Online ISBN: 978-1-59259-565-5

  • eBook Packages: Springer Protocols

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