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Isolation of candidate cDNAs for portions of the Duchenne muscular dystrophy gene

Nature volume 323, pages 646–650 (1986)Cite this article

Abstract

Duchenne muscular dystrophy (DMD) and the less severe Becker muscular dystrophy (BMD) are human X-linked muscle-wasting disorders that have been localized to the band Xp21 by genetic linkage analysis1–9 and cytologically detectable abnormalities10–12. A cloned DNA segment, DXS164 (or pERT87), has been shown to detect deletions in the DNA of unrelated DMD and BMD males13–15. Here we present the nucleotide sequence of two highly conserved DNA fragments from the DXS164 locus and their homologous sequences from the mouse X chromosome. One of the human conserved segments hybridized to a large transcript in RNA isolated from human fetal skeletal muscle and was used to isolate cDNA clones which cover approximately 10% of this transcript. The cDNA clones map to Xp21 and hybridize with a minimum of eight small regions that span 130 kilobases (kb) of the DXS164 locus. These expressed sequences are candidates for portions of the gene responsible for both DMD and BMD.

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References

  1. Davies, K. E. et al. Nucleic Acids Res. 11, 2303–2312 (1983).

    Article  ADS  CAS  PubMed  PubMed Central  Google Scholar 

  2. Aldridge, J. et al. Am. J. hum. Genet. 36, 546–564 (1984).

    CAS  PubMed  PubMed Central  Google Scholar 

  3. deMartinville, B. et al. Am. J. hum. Genet. 37, 235–249 (1985).

    CAS  Google Scholar 

  4. Bakker, E. et al. Lancet ii, 655–658 (1985).

    Article  Google Scholar 

  5. Kingston, H. M. et al. Hum. Genet. 67, 6–17 (1984).

    Article  CAS  PubMed  Google Scholar 

  6. Brown, C. S. et al. Hum. Genet. 71, 62–74 (1985).

    Article  CAS  PubMed  Google Scholar 

  7. Dorkins, H. et al. Hum. Genet. 71, 103–107 (1985).

    Article  CAS  PubMed  Google Scholar 

  8. Fadda, S. et al. Hum. Genet. 71, 33–36 (1985).

    Article  CAS  PubMed  Google Scholar 

  9. Goodfellow, P. et al. Cytogenet. Cell Genet. 40, 296–352 (1985).

    Article  CAS  PubMed  Google Scholar 

  10. Boyd, Y. & Buckle, V. J. Clin. Genet. 29, 108–115 (1986).

    Article  CAS  PubMed  Google Scholar 

  11. Francke, U. et al. Am. J. hum. Genet. 37, 250–267 (1985).

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Ray, P. N. et al. Nature 318, 672–675 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  13. Kunkel, L. M. et al. Proc. natn. Acad. Sci. U.S.A. 82, 4778–4782 (1985).

    Article  ADS  CAS  Google Scholar 

  14. Monaco, A. P. et al. Nature 316, 842–845 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  15. Kunkel, L. M. et al. Nature 322, 73–77 (1986).

    Article  ADS  CAS  PubMed  Google Scholar 

  16. Frischauf, A. M. et al. J. molec. Biol. 170, 827–842 (1983).

    Article  CAS  PubMed  Google Scholar 

  17. Mount, S. M. Nucleic Acids Res. 10, 459–472 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Ruskin, B. et al. Cell 38, 317–331 (1984).

    Article  CAS  PubMed  Google Scholar 

  19. Keller, E. B. & Noon, N. A. Proc. natn. Acad. Sci. U.S.A. 81, 7417–7420 (1984).

    Article  ADS  CAS  Google Scholar 

  20. Knott, T. J. et al. Science 230, 37–43 (1985).

    Article  ADS  CAS  PubMed  Google Scholar 

  21. Breitbart, R. E. et al. Cell 41, 67–82 (1985).

    Article  CAS  PubMed  Google Scholar 

  22. Michelson, A. M. et al. Proc. natn. Acad. Sci. U.S.A. 80, 472–476 (1983).

    Article  ADS  CAS  Google Scholar 

  23. Gubler, U. & Hoffman, B. J. Gene 25, 263–269 (1983).

    Article  CAS  PubMed  Google Scholar 

  24. Young, R. A. & Davis, R. W. Science 222, 778–782 (1983).

    Article  ADS  CAS  PubMed  Google Scholar 

  25. Benton, W. D. & Davis, R. W. Science 196, 180–182 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

  26. Moser, H. Hum. Genet. 66, 17–40 (1984).

    Article  CAS  PubMed  Google Scholar 

  27. Fischbeck, K. et al. Lancet ii, 104 (1986).

    Article  Google Scholar 

  28. Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning, a Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  29. Bolivar, F. et al. Gene 2, 95–113 (1977).

    Article  CAS  PubMed  Google Scholar 

  30. Vierra, J. & Messing, J. Gene 19, 259 (1982).

    Article  Google Scholar 

  31. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  PubMed  Google Scholar 

  32. Bruns, G. A. P. et al. Adv. exp. Med. Biol. 154, 60–72 (1982).

    Google Scholar 

  33. Sanger, F. et al. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  34. Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201–5205 (1980).

    Article  ADS  CAS  Google Scholar 

  35. Chirgwin, J. M. et al. Biochemistry 18, 5294–5299 (1979).

    Article  CAS  PubMed  Google Scholar 

  36. Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).

    Article  ADS  CAS  Google Scholar 

  37. Singer, M. F. Cell 28, 433–434 (1982).

    Article  CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Division of Genetics, Mental Retardation Program, Department of Pediatrics, Harvard Medical School, The Children's Hospital, Boston, Massachusetts, 02115, USA

    Anthony P. Monaco, Rachael L. Neve, Chris Colletti-Feener, Corlee J. Bertelson, David M. Kurnit & Louis M. Kunkel

  2. The Program in Neuroscience, Harvard University, Cambridge, Massachusetts, 02138, USA

    Anthony P. Monaco, Rachael L. Neve & Louis M. Kunkel

Authors
  1. Anthony P. Monaco
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  2. Rachael L. Neve
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  3. Chris Colletti-Feener
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  4. Corlee J. Bertelson
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  5. David M. Kurnit
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  6. Louis M. Kunkel
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Monaco, A., Neve, R., Colletti-Feener, C. et al. Isolation of candidate cDNAs for portions of the Duchenne muscular dystrophy gene. Nature 323, 646–650 (1986). https://doi.org/10.1038/323646a0

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