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Purified λ regulatory protein cII positively activates promoters for lysogenic development

Nature volume 292, pages 128–132 (1981)Cite this article

Abstract

The bacteriophage λ regulatory protein, cII, has been purified and shown to activate positively RNA transcription from the two phage promoters which coordinately regulate phage lysogenic development. To obtain this protein, the cII gene was cloned into a plasmid vector carrying the strong, regulatable λ phage promoter PL such that it was overproduced to levels approaching 5% of cellular protein.

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Authors and Affiliations

  1. Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20205, USA

    Hiroyuki Shimatake & Martin Rosenberg

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  1. Hiroyuki Shimatake
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  2. Martin Rosenberg
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Shimatake, H., Rosenberg, M. Purified λ regulatory protein cII positively activates promoters for lysogenic development. Nature 292, 128–132 (1981). https://doi.org/10.1038/292128a0

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  • DOI: https://doi.org/10.1038/292128a0

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