CD Genomics provides the most cost-effective high-throughput DNA sequencing services with a focus on Shotgun Library Sequencing, cDNA Library Sequencing, SAGE Library Sequencing, Whole Genome Sequencing and Primer Walking Sequencing.

One widely used strategy for DNA sequencing is based on analysis of large DNA molecules by primer walking (4).

We used primer walking PCR and Sanger sequencing to fill the gaps in the genome (online Technical Appendix Figure 2).

Allele-specific plasmids were sequenced by primer walking.

We determined the nearly complete genome sequence of BuV, which we named Mpulungu BuV (MpBuV), by filling genome gaps with primer walking and rapid amplification of cDNA ends (GenBank/EMBL/DDBJ accession no.